human gbm cell line u 87 mg (ATCC)
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human gbm cell line u 87 mg
Human Gbm Cell Line U 87 Mg, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u+87+mg+cell+lines/U-87+MG/pmc13156593-197-2-8
Average 99 stars, based on 10512 article reviews
Human Gbm Cell Line U 87 Mg, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u+87+mg+cell+lines/U-87+MG/pmc13156593-197-2-8
Average 99 stars, based on 10512 article reviews
human gbm cell line u 87 mg - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "AAV transduction of human glioblastoma cell lines induces perturbation in cell proliferation"
Article Title: AAV transduction of human glioblastoma cell lines induces perturbation in cell proliferation
Journal: Molecular Therapy Oncology
doi: 10.1016/j.omton.2026.201216
Figure Legend Snippet: Differential transduction of human GBM cell lines with AAV vector serotypes (A) Representative images of U-87 MG and patient-derived cell lines transduced with AAV1, 2, 1/2, 6, and 6.2 expressing eGFP and pBS control-treated control. Green, GFP expression; magenta, EdU staining; blue, Hoechst staining. Scale bars, 100 μm. (B) Quantitation of the transduction efficiency for all serotypes in each cell line as determined by GFP expression. All data presented as mean (SD), n = 3 biological replicates.
Techniques Used: Transduction, Plasmid Preparation, Derivative Assay, Expressing, Control, Staining, Quantitation Assay
Figure Legend Snippet: AAV transduced CD44-positive cell populations with greater efficiency (A) Transduction efficiency of AAV1, 2, 1/2, 6, and 6.2 in CD44-positive and -negative cell populations. Two-way ANOVA showed no significant interaction between the effects of AAV serotype and CD44 expression on transduction efficiency in all cell lines. There was a significant main effect of CD44 expression in U-87 MG ( p = 0.007), case 1 ( p = 0.0003), and case 4 ( p = 0.010) but no statistical significance was found following Sidak’s multiple comparisons test. (B) CD44 expression in human GBM cell lines following AAV treatment. (C) Proliferation of the CD44-positive cell population normalized to values from NTC-treated control. Treatments were compared against their respective pBS-treated control. All data presented as mean (SD), n = 3 biological replicates. Statistical significance is shown as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Techniques Used: Transduction, Expressing, Control
Figure Legend Snippet: Reductions in cell number and proliferation following AAV treatment persisted in the absence of transgene expression (A) Representative images of U-87 MG, cases 2 and 5 transduced with AAV expressing eGFP or no transgene, along with their respective pBS controls. Green, GFP expression; magenta, EdU staining; blue, Hoechst staining. Scale bars, 100 μm. (B) Total cell number and (C) cell proliferation in the 24 h prior to fixation, normalized to values from NTC-treated control. Treatments were compared against their respective pBS-treated control. All data presented as mean (SD), n = 3 biological replicates. Statistical significance is shown as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Techniques Used: Expressing, Transduction, Staining, Control
Figure Legend Snippet: AAV treatment induced cell-cycle arrest at G1 in cases 2 and 5 but not U-87 MG (A) Cumulative cytotoxicity was evaluated by LDH release assay 4 days following AAV treatment, normalized to the maximal LDH release from the Triton X-100 control. No statistical significance was detected. (B) Percentage of cells in each cell cycle phase (G0/G1, S, and G2/M) were quantified via DNA content profiling (see also ). All data presented as mean (SD), n = 3 biological replicates. Treatments were compared against their respective pBS-treated control. Statistical significance is shown as G0/G1 phases: ∗∗∗∗ p < 0.0001; S phase: † p < 0.05; G2/M phases: # p < 0.05, ### p < 0.001.
Techniques Used: Lactate Dehydrogenase Assay, Control
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